Ochratoxins (Total / A+B+C), ELISA, 96-test

Immunoassay for the detection of total Ochratoxins (A/B/C).

The Ochratoxins ELISA is an immunoassay for the detection of total Ochratoxins (A/B/C). This test is suitable for the quantitative and/or qualitative detection of Ochratoxins in contaminated samples. Positive samples should be confirmed by HPLC, GC/MS, or other conventional methods.

The test is a direct competitive ELISA based on the recognition of Ochratoxins by specific antibodies. Ochratoxins, when present in a sample and a Ochratoxin-enzyme conjugate compete for the binding sites of anti-Ochratoxins antibodies which are immobilized on the wells of the microtiter plate. After a washing step and addition of the substrate solution, a color signal is produced. The intensity of the blue color is inversely proportional to the concentration of Ochratoxins present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.