B ZERO® DON v2, ELISA, 96 det.

B ZERO® DON v2, ELISA, 96 det.

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ELISA test kit for the quantitative detection of deoxynivalenol in cereals, feed and DDGS (Distillers).

Advantages: B ZERO® is the most cost-effective line of ELISA test kits. Thanks to the removal of the calibrators, the analysts are not required to use wells for the standard curve. Quantitative results are obtained by means of a virtual master-curve that is provided within each kit batch.

SKU HU0040008
Assay principle Microplate competitive enzyme immunoassay without standard curve.  Sample quantification through interpolation of inhibition value on virtual curve (master-curve) provided for each batch of kit.
Format Microtiter plate with 96 wells
Assay time 20 min (sample preparation not included)
Analysable samples Cereals, feed, DDGS, wheat bran and middlings
Detection limit (LOD)
  • Wheat, Maize, Feed and DDGS - 0.04 ppm
  • Durum Wheat - 0.12 ppm
  • Wheat Bran and Middlings - 0.24 ppm
Sample preparation
  • Cereals (Maize, Wheat), Feed, DDGS - grinding, methanol-water extraction, filtration
  • Durum Wheat - grinding, water extraction, centrifugation, dilution
  • Wheat Bran and Middlings - water extraction, activated charcoal clean-up, filtration, dilution
Specificity
  • Deoxynivalenol 100%
  • 3-acetyl-deoxynivalenol >100%
  • 3-glucosyl-deoxynivalenol 64%
  • 15-acetyl-deoxynivalenol 2%
  • Nivalenol <4%