B ZERO® AFLA B1, ELISA, 48 det.

B ZERO® AFLA B1, ELISA, 48 det.

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ELISA test kit for the quantitative detection of aflatoxin B1.

Advantages: B ZERO® is the most cost-effective line of ELISA test kits. Thanks to the removal of the calibrators, the analysts are not required to use wells for the standard curve. Quantitative results are obtained by means of a virtual master-curve that is provided within each kit batch.

SKU HU0040025
Assay principle Microplate competitive enzyme immunoassay without standard curve. Sample quantification through interpolation of inhibition value on virtual curve (master-curve) provided for each batch of kit.
Format Microtiter plate with 48 wells
Assay time 15 min
Analysable samples Cereals (sorghum, corn, high moisture corn, brown rice), corn gluten meal, silage and mash, feed, nuts (hazelnuts, pistachios, peanuts, almonds), peanut meal, dried fruits (raisins, figs), DDGS, cottonseed, soy, soybean meal.
Detection limit (LOD) 1 ppb (for all the matrices)
Sample preparation
  • Grinding, methanol - water extraction, filtration.
  • For Silage and mash: pH adjustment of methanol extracts.
  • For Cottonseed: grinding and homogenization, methanol - water extraction, sedimentation.
Specificity
  • Aflatoxin B1 100%
  • Aflatoxin B2 5%
  • Aflatoxin B1 19%
  • Aflatoxin B1 < 1%